Analysis of grande and petite mitochondrial DNA by DNA-DNA hybridization
نویسندگان
چکیده
منابع مشابه
Mitochondrial DNA Mutations, Pathogenicity and Inheritance
Mitochondria contain their own DNA (mtDNA), which codes for 13 proteins (all subunits of the respiratory chain complexes), 22 tRNAs and 2 rRNAs. Several mtDNA point mutations as well as deletions have been shown to be causative in well-defined mitochondrial disorders. A mixture of mutated and wild type mtDNA (heteroplasmy) is found in most of these disorders. Inheritance of mtDNA is maternal, a...
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Digestion of grande mitochondrial DNA (mtDNA) BY EcoRI restriction endonuclease gives rise to nine fragments with a total molecular weight of 51.8 x 10(6). HindIII digestion yields six fragments with a similar total molecular weight. Specific restriction fragments can be detected despite the fact that yeast mtDNA consists of a heterogeneous distribution of randomly broken molecules. Digestion p...
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Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...
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Resolving the phylogeny of the hominoid primates is a test case for molecular systematic methods because, by most genetic measures, humans, chimpanzees, and gorillas are very closely related. Recently, Sibley and Ahlquist (1984) applied DNADNA hybridization techniques to infer the hominoid phylogenetic tree with humans and chimpanzees closest. Templeton (1983, 1985) argues rather that the chimp...
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OBJECTIVE The purpose of this investigation was to examine the microbiota of infected root canals by using a molecular genetic method. STUDY DESIGN The presence and levels of 42 bacterial species were determined in 28 root canal samples by using whole genomic DNA probes and checkerboard DNA-DNA hybridization. To confirm the presence of bacterial DNA in clinical samples, a polymerase chain rea...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1972
ISSN: 0014-5793
DOI: 10.1016/0014-5793(72)80740-3